īongers T, Ferris H (1999) Nematode community structure as a bioindicator in environmental monitoring. īokulich NA, Mills DA (2013) Improved selection of internal transcribed spacer-specific primers enables quantitative, ultra-high-throughput profiling of fungal communities. īesnard G, Christin PA, Malé PJG, Lhuillier E, Lauzeral C, Coissac E, Vorontsova MS (2014) From museums to genomics: Old herbarium specimens shed light on a C3 to C4 transition. īellemain E, Carlsen T, Brochmann C, Coissac E, Taberlet P, Kauserud H (2010) ITS as an environmental DNA barcode for fungi: An in silico approach reveals potential PCR biases. campestris strains suggests the evolution of local endemic populations of the pathogen and does not correlate with race distribution. īella P, Moretti C, Licciardello G, Strano CP, Pulvirenti A, Alaimo S, Zaccardelli M, Branca F, Buonaurio R, Vicente JG, Catara V (2019) Multilocus sequence typing analysis of Italian Xanthomonas campestris pv. īecker S, Hanner R, Steinke D (2011) Five years of FISH-BOL: Brief status report. ![]() īanchi E, Ametrano CG, Stanković D, Verardo P, Moretti O, Gabrielli F, Lazzarin S, Borney MF, Tassan F, Tretiach M, Pallavicini A, Muggia L (2018) DNA metabarcoding uncovers fungal diversity of mixed airborne samples in Italy. J Microbiol Methods 55(3):541–555īalajee SA, Borman AM, Brandt ME, Cano J, Cuenca-Estrella M, Dannaoui E, Guarro J, Haase G, Kibbler CC, Meyer W, O’donnell K, Petti CA, Rodriguez-Tudela JL, Sutton D, Velegraki A, Wickes BL, (2009) Sequence-based identification of Aspergillus, Fusarium, and Mucorales species in the clinical mycology laboratory: where are we and where should we go from here? J Clin Microbiol 47(4):877–884. īaker GC, Smith JJ, Cowan DA (2003) Review and re-analysis of domain-specific 16S primers. Īvó AP, Daniell TJ, Neilson R et al (2017) DNA barcoding and morphological identification of benthic nematodes assemblages of estuarine intertidal sediments: Advances in molecular tools for biodiversity assessment. Īrahal DR, Sánchez E, Macián Rovira MC, Garay Auban E (2008) Value of recN sequences for species identification and as a phylogenetic marker within the family “Leuconostocaceae.” Int Microbiol 11(1):33–39. Īlkowni R, Alabdallah O, Fadda Z (2019) Molecular identification of tomato brown rugose fruit virus in tomato in Palestine. Īli MM, Li F, Zhang Z, Zhang K, Kang DK, Ankrum JA, Le XC, Zhao W (2014) Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine. ![]() Ībeysinghe S, Abeysinghe PD, Kanatiwela-de Silva C, Udagama P, Warawichanee K, Aljafar N, Kawicha P, Dickinson M (2016) Refinement of the taxonomic structure of 16SrXI and 16SrXIV phytoplasmas of gramineous plants using multilocus sequence typing. ![]() Supplementary indexing primers are also available, which increase multiplexing capacity to 24 samples.Īlternatively, you can send Cellecta cells, tissues, or genomic DNA and have us prepare, sequence and analyze the samples for you.Abdelfattah A, Wisniewski M, Droby S, Schena L (2016) Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase. Each kit contains sufficient primers and reagents for 6-48 samples (48 preps of 50 ug each, 12 multiplex). ![]() Primers are specific to each library/vector combination, so you simply need to choose a kit appropriate for the library you are using. Our service includes the DNA preparation, sequencing run, and demultiplexing/alignment, so you receive a spreadsheet with reads/guide/sample for each element in the library, with optional DNA isolation from cells or tissue samples.Ĭellecta NGS Analysis Kits provide the enzymes, reagents, and 15 indexed amplification primers used to prepare sequencing libraries for multiplex NGS analysis. Use of a pooled lentiviral CRISPR, shRNA, or barcode library in an experiment requires amplification and next-generation sequencing (NGS) analysis of the barcodes or guides from genomic DNA isolated from cells transduced with the library.
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